Composite
Part:BBa_K1905003:Design
Designed by: Juan Carlos Rueda Silva Group: iGEM16_TecCEM_HS (2016-09-13)
Riboswitch to detect E5 mRNA from HPV-16 and express RFP
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 838
Illegal AgeI site found at 950 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
The main design consideration is the proper fold of the mRNA and hybridization with E5 mRNA.
Source
It does not come from a genomic sequence, it assembled and engineered in order to fold and unfold in presence or absence of E5 mRNA and code RFP.